Studies on Imaging System and Machine Learning: 3D Halftoning and Human Facial Landmark Localization

In this dissertation, studies on digital halftoning and human facial landmark localization will be discussed. 3D printing is becoming increasingly popular around the world today. By utilizing 3D printing technology, customized products can be manufactured much more quickly and efficiently with much less cost. However, 3D printing still suffers from low-quality surface reproduction compared with 2D printing. One approach to improve it is to develop an advanced halftoning algorithm for 3D printing. In this presentation, we will describe a novel method to 3D halftoning that can cooperate with 3D printing technology in order to generate a high-quality surface reproduction. In the second part of this report, a new method named direct element swap to create a threshold matrix for halftoning is proposed. This method directly swaps the elements in a threshold matrix to find the best element arrangement by minimizing a designated perceived error metric. Through experimental results, the new method yields halftone quality that is competitive with the conventional level-by-level matrix design method. Besides, by using direct element swap method, for the first time, threshold matrix can be designed through being trained with real images. In the second part of the dissertation, a novel facial landmark detection system is presented. Facial landmark detection plays a critical role in many face analysis tasks. However, it still remains a very challenging problem. The challenges come from the large variations of face appearance caused by different illuminations, different facial expressions, different yaw, pitch and roll angles of heads and different image qualities. To tackle this problem, a novel coarse-to-fine cascaded convolutional neural network system for robust facial landmark detection of faces in the wild is presented. The experiment result shows our method outperforms other state-of-the-art methods on public test datasets. Besides, a frontal and profile landmark localization system is proposed and designed. By using a frontal/profile face classifier, either frontal landmark configuration or profile landmark configuration is employed in the facial landmark prediction based on the input face yaw angle

Consensus Recommendation for Mouse Models of Ocular Hypertension to Study Aqueous Humor Outflow and Its Mechanisms.

Due to their similarities in anatomy, physiology, and pharmacology to humans, mice are a valuable model system to study the generation and mechanisms modulating conventional outflow resistance and thus intraocular pressure. In addition, mouse models are critical for understanding the complex nature of conventional outflow homeostasis and dysfunction that results in ocular hypertension. In this review, we describe a set of minimum acceptable standards for developing, characterizing, and utilizing mouse models of open-angle ocular hypertension. We expect that this set of standard practices will increase scientific rigor when using mouse models and will better enable researchers to replicate and build upon previous findings

Studies on Imaging Systems and Machine Learning: 3D Halftoning and Human Facial Landmark Localization

In this dissertation, studies on digital halftoning and human facial landmark localization will be discussed. 3D printing is becoming increasingly popular around the world today. By utilizing 3D printing technology, customized products can be manufactured much more quickly and efficiently with much less cost. However, 3D printing still suffers from low-quality surface reproduction compared with 2D printing. One approach to improve it is to develop an advanced halftoning algorithm for 3D printing. In this presentation, we will describe a novel method to 3D halftoning that can cooperate with 3D printing technology in order to generate a high-quality surface reproduction. In the second part of this report, a new method named direct element swap to create a threshold matrix for halftoning is proposed. This method directly swaps the elements in a threshold matrix to find the best element arrangement by minimizing a designated perceived error metric. Through experimental results, the new method yields halftone quality that is competitive with the conventional level-by-level matrix design method. Besides, by using direct element swap method, for the first time, threshold matrix can be designed through being trained with real images. In the second part of the dissertation, a novel facial landmark detection system is presented. Facial landmark detection plays a critical role in many face analysis tasks. However, it still remains a very challenging problem. The challenges come from the large variations of face appearance caused by different illuminations, different facial expressions, different yaw, pitch and roll angles of heads and different image qualities. To tackle this problem, a novel coarse-to-fine cascaded convolutional neural network system for robust facial landmark detection of faces in the wild is presented. The experiment result shows our method outperforms other state-of-the-art real-time methods on public test datasets. Besides, a frontal and profile landmark localization system is proposed and designed. By using a frontal/profile face classifier, either frontal landmark configuration or profile landmark configuration is employed in the facial landmark prediction based on the input face yaw angle

A comparative UPLC-Q-Orbitrap-MS untargeted metabolomics investigation of different parts of Clausena lansium (Lour.) Skeels

In this study, the non-targeted large-scale plant metabolomics (UPLC-Q-Orbitrap-MS) was performed for the comparison of chemical profiling of the leaves, barks, flowers, peels, pulps, and seeds of Clausena lansium (Lour.) Skeels (called “wampee”). A total of 364 metabolites were identified, and 62 potential biomarkers were selected by the multivariate statistical analysis. Hierarchical cluster analysis suggested that the selected biomarkers were significant differential metabolites among various parts of wampee. Metabolic pathway analysis showed a significant enrichment of the “Flavone and flavonol synthesis” and “Isoquinoline alkaloid biosynthesis” pathway. This study provides important information for the isolation and identification of functional components from different tissues of wampee and the metabolic biosynthesis pathway elucidation in detail